fairplay™ microarray labeling kit (Agilent technologies)
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Agilent technologies
fairplay™ microarray labeling kit
Fairplay™ Microarray Labeling Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fairplay+kit/pmc00179896-192-11-15
Average 90 stars, based on 1 article reviews
Fairplay™ Microarray Labeling Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fairplay+kit/pmc00179896-192-11-15
Average 90 stars, based on 1 article reviews
fairplay™ microarray labeling kit - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)"
Article Title: Sensitivity and fidelity of DNA microarray improved with integration of Amplified Differential Gene Expression (ADGE)
Journal: BMC Genomics
doi: 10.1186/1471-2164-4-28
Figure Legend Snippet: Composite images of ADGE microarray and regular microarray. The clones corresponding to the contiguous area of twelve spots (3 × 4) were amplified by using PCR with the primers having a Taq I site at the end. After cut with Taq I , the same amount of DNA for each clone was ligated to the CT and TT adapters. The CT and TT adapter-linked DNA fragments were mixed in ratios of 1:1 for the three clones of the first column, 1:2 for the clones of the second column, 1:3 for the clones of the third column, 1:4 for the clones of the fourth column. The top panel is the result of ADGE microarray while the bottom panel is the result of regular microarray. The ratios are represented by Cy5 (green) to Cy3 (red) and normalized with the value of clones in the first column. The detected ratios are averages of three spots in each column.
Techniques Used: Microarray, Clone Assay, Amplification
Figure Legend Snippet: Relationship between detected ratios (y) and input ratios (x). The relationship is y = 1.05x 1.55 with R 2 = 0.97 for ADGE microarray while it is y = 0.56x +0.39 with R 2 = 0.96 for regular microarray within the input ratio of 4 (panel A). Within the input ratio range of 1~20, the relationship is y = 15.99ln(x) - 6.14 with R 2 = 0.84 for ADGE-microarray (panel B).
Techniques Used: Microarray
Figure Legend Snippet: The MA plots of ADGE microarray and regular microarray. A is the average of log 2 Cy5 and log 2 Cy3, representing intensities of spots. M is the difference of log 2 Cy5 and log 2 Cy3, representing the expression ratios in the power of 2, with positive values for up-regulated genes, negative values for down-regulated genes and 0 for unchanged genes. Panel A: ADGE microarray with HL60 vs HL60/TLK286, average of three replicates. Panel B: regular microarray with HL60 vs HL60/TLK286, average of three replicates. Panel C: ADGE microarray with HL60 vs HL60, average of two replicates. Panel D: number of differential genes selected from all genes on the chip or from genes with > 99% confidence level.
Techniques Used: Microarray, Expressing
Figure Legend Snippet: The variances of Cy3 (HL60) (panel A) and Cy5 (HL60/TLK286) (panel B) in ADGE microarray and regular microarray. The normalized values of Cy3 and Cy5 were used to calculate the variances. Panel C is the number of genes with confidence levels of 90% or greater in ADGE microarray and regular microarray. The confidence level is a result of t-test for each gene.
Techniques Used: Microarray
Figure Legend Snippet: Comparison of ADGE microarray results between two duplicated spots
Techniques Used: Microarray
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Microarray analysis of gene expression cDNA, generated from 20 μg RNA by Labeling:Article Title: Tamoxifen Induces Expression of Immune Response-Related Genes in Cultured Normal Human Mammary Epithelial Cells Article Snippet: cDNA, generated from 20 μg RNA by Fairplay Kit (Stratagene, Cedar Grove,TX), was labeled with Cy3 (unexposed control) and Cy5 (TAM-exposed) by indirect coupling, denatured and hybridized to Hs-Operon v2-vB1 oligoarrays containing over 20,000 immobilized human gene elements (Microarray Facility, Advanced Technology Center, NCI). .. cDNA, generated from 20 μg RNA by Article Title: Tamoxifen Induces Expression of Immune Response-Related Genes in Cultured Normal Human Mammary Epithelial Cells Article Snippet: Microarray analysis of gene expression cDNA, generated from 20 μg RNA by Fairplay Kit (Stratagene, Cedar Grove,TX), was labeled with Cy3 (unexposed control) and Cy5 (TAM-exposed) by indirect coupling, denatured and hybridized to Hs-Operon v2-vB1 oligoarrays containing over 20,000 immobilized human gene elements (Microarray Facility, Advanced Technology Center, NCI). .. Microarray analysis of gene expression cDNA, generated from 20 μg RNA by Article Title: Gene expression profiles of human breast cancer progression Article Snippet: Sequence-verified human cDNA clones were obtained from Research Genetics (Huntsville, AL). cDNA inserts were amplified by PCR, purified, and spotted onto a 1 × 3-inch SuperAmine (TeleChem International, Sunnyvale, CA) glass microscope slide by using an OmniGrid robotic arrayer (GeneMachines, San Carlos, CA). .. 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RNA was prepared from 20 ml of M. tuberculosis cultures as described above and subsequently treated with QIAGEN’s RNAeasy RNA clean-up protocol. cDNA libraries were made using the Hybridization:Article Title: Gene expression profiles of human breast cancer progression Article Snippet: Sequence-verified human cDNA clones were obtained from Research Genetics (Huntsville, AL). cDNA inserts were amplified by PCR, purified, and spotted onto a 1 × 3-inch SuperAmine (TeleChem International, Sunnyvale, CA) glass microscope slide by using an OmniGrid robotic arrayer (GeneMachines, San Carlos, CA). .. Probe Labeling and Hybridization. cDNA was transcribed from aRNA in the presence of 5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aminoallyl dUTP) by using |
